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recombinant il 40  (Aviva Systems)


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    Structured Review

    Aviva Systems recombinant il 40
    Recombinant Il 40, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+il+40/IL-9+Recombinant+Protein/pm40457473-176-16-22
    Average 93 stars, based on 1 article reviews
    recombinant il 40 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    In Vitro:

    Article Title: New pro-inflammatory cytokine IL-40 is produced by activated neutrophils and plays a role in the early stages of seropositive rheumatoid arthritis
    Article Snippet: All isolated neutrophils were transferred into 6-well plates (1×10 6 per well) in RPMI-1640 culture medium (Thermo Fisher) containing 10% heat-inactivated fetal bovine serum (Biowest, Nuaillé, France), 0.2% GlutaMax and 0.2% HEPES solution (both from Sigma-Aldrich, St. Louis, MO, USA). .. To assess the effect of extracellular IL-40 on neutrophils in vitro, neutrophils were stimulated with recombinant IL-40 (10, 50, 100 and 250 ng/mL, Aviva Systems Biology, San Diego, CA, USA) and with lipopolysaccharide as a positive control (LPS, 100 ng/mL, LPS from E. coli O26:B6, Sigma-Aldrich) for an interval of 4 hours and/or 24 hours at 37°C in a humidified atmosphere of 5% CO 2 . .. Simultaneously, to induce NETosis, neutrophils were treated with phorbol 12-myristate-13-acetate (PMA; 100 nM, Sigma-Aldrich) for 4 hours under the conditions described above.

    Recombinant:

    Article Title: New pro-inflammatory cytokine IL-40 is produced by activated neutrophils and plays a role in the early stages of seropositive rheumatoid arthritis
    Article Snippet: All isolated neutrophils were transferred into 6-well plates (1×10 6 per well) in RPMI-1640 culture medium (Thermo Fisher) containing 10% heat-inactivated fetal bovine serum (Biowest, Nuaillé, France), 0.2% GlutaMax and 0.2% HEPES solution (both from Sigma-Aldrich, St. Louis, MO, USA). .. To assess the effect of extracellular IL-40 on neutrophils in vitro, neutrophils were stimulated with recombinant IL-40 (10, 50, 100 and 250 ng/mL, Aviva Systems Biology, San Diego, CA, USA) and with lipopolysaccharide as a positive control (LPS, 100 ng/mL, LPS from E. coli O26:B6, Sigma-Aldrich) for an interval of 4 hours and/or 24 hours at 37°C in a humidified atmosphere of 5% CO 2 . .. Simultaneously, to induce NETosis, neutrophils were treated with phorbol 12-myristate-13-acetate (PMA; 100 nM, Sigma-Aldrich) for 4 hours under the conditions described above.

    Article Title: Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes.
    Article Snippet: The isolated PBMCs were seeded onto 6-well plates at a density of 1 × 106/mL in RPMI-1640 culture medium supplemented with 1% penicillin‒streptomycin solution (5,000 U/mL) (both from Thermo Fisher, Waltham, MA, USA). .. To assess the proinflammatory and profibrotic effects of extracellular IL-40, freshly isolated PBMCs were exposed to recombinant IL-40 (Recombinant Human Protein IL-40/C17orf99; Aviva Systems Biology, San Diego, USA) at concentrations ranging from 1 to 250 ng/mL. ..

    Article Title: IL-40 is up-regulated in the synovial fluid and cartilage of osteoarthritis patients and contributes to the alteration of chondrocytes phenotype in vitro
    Article Snippet: Chondrocytes were expanded according to the manufacturer's protocol and cultured in CBMTM Chondrocyte Growth Basal Medium (CC-3217, Lonza, Basel, Switzerland) supplemented with Chondrocyte ReagentPackTM Subculture Reagents (CC-4409, Lonza, Basel, Switzerland) at 37°C in a 5% CO2 humidified atmosphere. .. Cells in the second passage were seeded on 6-well plates (1.0 × 10 5 cells/well) and exposed to recombinant IL-40 (Aviva Systems Biology, San Diego, CA, USA) at concentrations ranging from 5 to 200 ng/ml for 24 hours (n=7-9). ..

    Article Title: Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes
    Article Snippet: The isolated PBMCs were seeded onto 6-well plates at a density of 1 × 10 6 /mL in RPMI-1640 culture medium supplemented with 1% penicillin‒streptomycin solution (5,000 U/mL) (both from Thermo Fisher, Waltham, MA, USA). .. To assess the proinflammatory and profibrotic effects of extracellular IL-40, freshly isolated PBMCs were exposed to recombinant IL-40 (Recombinant Human Protein IL-40/C17orf99; Aviva Systems Biology, San Diego, USA) at concentrations ranging from 1 to 250 ng/mL. ..

    Positive Control:

    Article Title: New pro-inflammatory cytokine IL-40 is produced by activated neutrophils and plays a role in the early stages of seropositive rheumatoid arthritis
    Article Snippet: All isolated neutrophils were transferred into 6-well plates (1×10 6 per well) in RPMI-1640 culture medium (Thermo Fisher) containing 10% heat-inactivated fetal bovine serum (Biowest, Nuaillé, France), 0.2% GlutaMax and 0.2% HEPES solution (both from Sigma-Aldrich, St. Louis, MO, USA). .. To assess the effect of extracellular IL-40 on neutrophils in vitro, neutrophils were stimulated with recombinant IL-40 (10, 50, 100 and 250 ng/mL, Aviva Systems Biology, San Diego, CA, USA) and with lipopolysaccharide as a positive control (LPS, 100 ng/mL, LPS from E. coli O26:B6, Sigma-Aldrich) for an interval of 4 hours and/or 24 hours at 37°C in a humidified atmosphere of 5% CO 2 . .. Simultaneously, to induce NETosis, neutrophils were treated with phorbol 12-myristate-13-acetate (PMA; 100 nM, Sigma-Aldrich) for 4 hours under the conditions described above.

    Isolation:

    Article Title: Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes.
    Article Snippet: The isolated PBMCs were seeded onto 6-well plates at a density of 1 × 106/mL in RPMI-1640 culture medium supplemented with 1% penicillin‒streptomycin solution (5,000 U/mL) (both from Thermo Fisher, Waltham, MA, USA). .. To assess the proinflammatory and profibrotic effects of extracellular IL-40, freshly isolated PBMCs were exposed to recombinant IL-40 (Recombinant Human Protein IL-40/C17orf99; Aviva Systems Biology, San Diego, USA) at concentrations ranging from 1 to 250 ng/mL. ..

    Article Title: Serum IL-40 is elevated in systemic sclerosis and is linked to disease activity, gastrointestinal involvement, immune regulation and fibrotic processes
    Article Snippet: The isolated PBMCs were seeded onto 6-well plates at a density of 1 × 10 6 /mL in RPMI-1640 culture medium supplemented with 1% penicillin‒streptomycin solution (5,000 U/mL) (both from Thermo Fisher, Waltham, MA, USA). .. To assess the proinflammatory and profibrotic effects of extracellular IL-40, freshly isolated PBMCs were exposed to recombinant IL-40 (Recombinant Human Protein IL-40/C17orf99; Aviva Systems Biology, San Diego, USA) at concentrations ranging from 1 to 250 ng/mL. ..



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    Image Search Results


    CD19-positive exosomes activate CAR T cells. A IFN-γ and IL-2 secretion by CD19-CAR T cells cultured in the presence of increasing concentrations of CD19 + or CD19 − EVs. Analysis of IFN-γ and IL-2 secretion by CD19-CAR T cells in the absence of stimulation is shown as control. Data are presented as the mean ± SD of three experimental replicates in at least two independent experiments. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparison test. B Targeted multiplex analysis of CD19-CAR T cells (n = 2) treated with CD19 + or CD19 − EVs. Untreated CD19-CAR T cells were used as a control. Heatmap represents genes with highest (red) and lowest (blue) expression ( p value < 0.01, fold change > 1.5). Color key indicates the changes of normalized expression values.

    Journal: Scientific Reports

    Article Title: CAR-tropic extracellular vesicles carry tumor-associated antigens and modulate CAR T cell functionality

    doi: 10.1038/s41598-023-27604-5

    Figure Lengend Snippet: CD19-positive exosomes activate CAR T cells. A IFN-γ and IL-2 secretion by CD19-CAR T cells cultured in the presence of increasing concentrations of CD19 + or CD19 − EVs. Analysis of IFN-γ and IL-2 secretion by CD19-CAR T cells in the absence of stimulation is shown as control. Data are presented as the mean ± SD of three experimental replicates in at least two independent experiments. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparison test. B Targeted multiplex analysis of CD19-CAR T cells (n = 2) treated with CD19 + or CD19 − EVs. Untreated CD19-CAR T cells were used as a control. Heatmap represents genes with highest (red) and lowest (blue) expression ( p value < 0.01, fold change > 1.5). Color key indicates the changes of normalized expression values.

    Article Snippet: Human T cells were activated with CD3/CD28 Dynabeads (Thermo Scientific, USA) at a 1:1 ratio (Life Technologies, USA) in a complete RPMI containing 40 IU/mL recombinant IL-2 (Pan Biotech, USA) for 24 h. Activated T cells were re-suspended at concentration of 0.5 × 10 6 /mL in lentiviral supernatant of CD19-CAR 1 mL of fresh RPMI media supplemented with 30 IU/ml IL-2 was added, and cells were plated to 6-well plates.

    Techniques: Cell Culture, Multiplex Assay, Expressing